By T.K. Ghose ,A. Fiechter, N.Blakebrough
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Additional info for Advances in Biochemical Engineering, Volume 003
R e c o m b i n a t i o n b e t w e e n loci f o u n d in different g r o u p s gave high yields o f r e c o m b i n a n t s with r e s t o r e d a n t i b i o t i c activity. O n the o t h e r hand, crossing of m u t a n t s b l o c k e d in t h e loci o f the s a m e g r o u p resulted in the f o r m a t i o n o f inactive r e c o m b i n a n t s (Table 8). This finding was c o n f i r m e d also d u r i n g r e c i p r o c a l crossing of different types of b l o c k e d m u t a n t s with the active strain (Table 9).
G e n e t i c R e c o m b i n a t i o n The existing data on genetic recombination of producers of tetracycline antibiotics may be divided into several principal groups. Preliminary results obtained by the application of the recombination technique for strain improvement have already been discussed in the first section. e. , 1972; Friend and Hopwood, 1971). , 1971b, 1972). Most of these investigations were conducted on the model of S. rimosus while data on the genome of S. , 1971b, 1972). Interspecies recombination between S.
A b b r e v i a t i o n s used: D T C , d e m e t h y l t e t r a c y c l i n e s : AVC, a u r e o v o c i n ; B-U, substances, the structure o f w h i c h has n o t been yet definitely established. "U ~= Metabolic type Table 5. Characteristics of s t a n d a r d strains o f S. , 1969b, 1971a) ~ :~ 9. o-~" g- p~ ~" ~,< Pz w 30 Z. HO~TALEKet al. 9- [~Z. 1- tnt~ Fluoreseention in UV-light: O yellow Q yellow green (]]) yeltow orange t~ brown Q red (~ orange O blue, blueviolet O violet Fig. 4. Chromatographic analysis of substances produced by pure and mixed cultures of blocked mutants of S.
Advances in Biochemical Engineering, Volume 003 by T.K. Ghose ,A. Fiechter, N.Blakebrough