New PDF release: Animal Cell Technology: Basic & Applied Aspects: Proceedings

By Takao Hayakawa (auth.), T. Kobayashi, Y. Kitagawa, K. Okumura (eds.)

ISBN-10: 9401043663

ISBN-13: 9789401043663

ISBN-10: 940110848X

ISBN-13: 9789401108485

Animal mobilephone know-how is a growing to be self-discipline of phone biology which goals to appreciate the constitution, functionality and behavior of differentiated animal cells, and particularly the advance of such skills as are priceless for commercial reasons. those advancements diversity from clonal growth of differentiated cells with precious talents, to optimization of phone tradition on business scale and modulation of the cells' talents to supply medicinal drugs and monoclonal antibodies.
The 6th quantity during this sequence offers a whole evaluate of contemporary cutting-edge in Japan, a rustic the place this box is principally good complicated. will probably be of curiosity to phone biologists, biochemists, molecular biologists, immunologists and different disciplines concerning animal mobile tradition, operating within the educational surroundings in addition to in (biotechnology or pharmaceutical) undefined.

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Additional resources for Animal Cell Technology: Basic & Applied Aspects: Proceedings of the Sixth International Meeting of the Japanese Association for Animal Cell Technology, Nagoya, Japan, November 9–12, 1993

Sample text

Berghei to mosquito Anopheles stephensi was strongly blocked in these mice. Immunoblotting analyses suggested that the recombinant Pbs21 produced in the silkworm larvae has a structure close to native Pbs21 and is a good candidate for a transmission blocking vaccine of the malaria parasite. 1. Introduction The baculovirus expression vector system using Autographa cali/ornica NPV (AcNPV) and Bombyx mod NPV (BmNPV) is now widely used for high level expression of foreign genes [I. 2, 3,41. Although a large-volume culture of the silkworm cell lines has not been established yet, BmNPV vector system has an unique advantageous characteristic such that it can be easily expanded from the in vitro system to an in vivo system using silkworm larvae for mass production of foreign gene products [51.

I. B. , 57, 272. , Suomela, H. and Ukkonen P. , 63, 6-11. , Aizawa, H. and Yamamoto, N. (1989) 'A novel method for removal of human immunodeficiency virus: filtration with porous polymeric membranes'. , 56, 230-236. , Yamamoto N. and Manabe S. (1989) 'Microparticie removability of regenerated cellulose hollow fibers (BMM)" J. , 38, 259. I. and Yamashizi, T. (1990) 'Structure of cuprammonium regenerated cellulose hollow fiber (BMM hollow fiber) for virus removal', Polym J, 22, 751-758. E. E(1992) 'High resolution removal of virus from protein solutions using a membrane of unique structure', Biotechnology, 10, 182-188.

Thereafter, high yields were consistently obtained. A similarly high yield was obtained even using protein free culture. 4 PURIFICATION OF RECOMBINANT hGHR-ED 234 Figure 2. Purification of the hGHR-ED form culture fluid of protein free mediulll. 1%-15%SDS PAGE and stained with Coomasie brilliant blue. M, Marker proteins. Lane, 1 Total protein of protein culture fluid; lane 2, Protein did not absorb to an hGH affinity column; lanes 3, Protein eluted from the hGH affinity column chromatography after adsorption; lane 4, sample from lane 3 concentrated 10fold.

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Animal Cell Technology: Basic & Applied Aspects: Proceedings of the Sixth International Meeting of the Japanese Association for Animal Cell Technology, Nagoya, Japan, November 9–12, 1993 by Takao Hayakawa (auth.), T. Kobayashi, Y. Kitagawa, K. Okumura (eds.)


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